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pegfp c1 expression vector  (Addgene inc)


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    Structured Review

    Addgene inc pegfp c1 expression vector
    Pegfp C1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+vector/pEGFP-C1-TBP+(Plasmid+%2326674)/pmc12880560-74-7-10
    Average 86 stars, based on 3 article reviews
    pegfp c1 expression vector - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
    Article Snippet: .. The following plasmids were used: mito-DsRed2 (a gift from T. Schwarz, Harvard Medical School), EGFP–FMRP (gift from G. Bassell, Emory University), GFP-POLG2 (gift from W. Copeland, NIH), Halo–FMRP (subcloned from EGFP–FMRP into pFN21A-HaloTag-CMV vector from Promega), COX8A–BFP (Goldsmith et al.49), LAMP1–Halo (Gallagher and Holzbaur50), GFP–MFF (Addgene, 49153), GFP-RAB7 (Addgene, 12605), GFP–RAB7 T22N (Addgene, 12660), RFP–RAB5 (Addgene, 14437), EGFP– DRP1 (subcloned from pcDNA 3.1-Drp1 (Addgene, 34706) into pEGFP-C1 vector), pCRISPRia-vs2 (Addgene, 84832), PGK 4xMito-mEmerald (Addgene, 200430), pUbC-OsTIR1-myc-IRES-scFv-sfGFP (Addgene, 84563), AID–SunTag–MFF (subcloned from GFP–MFF, MFF cDNA clone (Transomic BC000797) and pUbC-FLAG-24xSuntagV4-oxEBFPAID-baUTR1-24xMS2V5-Wpre (Addgene, 84561) into pEGFP-N1 vector with EGFP removed), EGFP–TWINKLE (subcloned from TWINKLE– APEX2-V5 (Addgene, 129705) into pEGFP-N1 vector), EGFP–TFAM (subcloned from pCellFree_G03 TFAM into pEGFP-N1 vector) and Halo–Stop (Cason et al. 51). ..

    Article Title: Endoplasmic reticulum exit sites are segregated for secretion based on cargo size.
    Article Snippet: The iLID fragment was originally obtained as a generous gift from the Kuhlman lab (Addgene 60411). .. TANGO1Short was amplified using 5’- GATCCGCTAGCGCTACCGGTGCCACCATGGACTCAGTACCTGCC-3’ and 5’-ACTACTACCACTACTACCTGGGCTCTGTTTTAAAGCCTG-3’, mCherry-iLID was amplified using 5’- GGTAGTAGTGGTAGTAGTATGGTGAGCAAGGGCGA-3’ and 5’- TCGAAGCTTGAGCTCGAGATCTTTAAAAGTAATTTTCGTCGTTCGCT3’.The fragments were inserted into a pEGFP-C1 vector (Addgene 46956) using the AgeI/BglII restriction sites. ..

    Article Title: Mycobacterium tuberculosis modulates NUDT21-mediated alternative polyadenylation to enhance FTH1 expression in macrophages and promotes intracellular growth
    Article Snippet: .. The 3′UTRs of FTH1 transcripts were cloned into a pEGFP-C1 vector (Addgene, Plasmid #36412) downstream of the GFP ORF to create 3′UTR-Long (L) and 3′UTR-Short (S) plasmids. ..

    Article Title: Endoplasmic reticulum exit sites are segregated for secretion based on cargo size.
    Article Snippet: .. The fragments were inserted into a pEGFP-C1 vector (Addgene 46956) using the AgeI/BglII restriction sites. ..

    Article Title: Endoplasmic reticulum exit sites are segregated for secretion based on cargo size.
    Article Snippet: .. The fragments were inserted into a pEGFP-C1 vector (Addgene 46956) using the PstI/BspEI restriction sites. ..

    Article Title: p53-dependent chromatin relaxation is required for DNA double-strand break repair.
    Article Snippet: .. The GFP-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a PEGFP-C1 vector (Addgene, Watertown, USA). .. The GST-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a pGEX-4T-2 vector (Addgene).

    Article Title: Plasma membrane rather than endosomal Gq signaling drives transcriptional activity by the viral chemokine receptor US28 in glioblastoma
    Article Snippet: C-tRFP-Lck cloned into PCMV6-AC-RFP expression vector was purchased from Origene (#RC100049). .. TagRFP-T-EEA1 in pEGFP-C1 vector and K44A HA-dynamin 1 in pcDNA3.1 were respective gifts from Silvia Corvera and Sandra Schmid (Addgene plasmids #42635 and #34683). β-arrestin1-GFP10 and rGFP-Rab7 in pcDNA3.1+ was a kind gift from Prof. Michel Bouvier (Université de Montréal, Canada) and Stéphane Laporte (McGill University, Canada). .. Renilla reniformis luciferase II (Rluc), US28-Rluc, and US28-PDT-Rluc in pcDNA3.1+ were synthetized by GenScript.

    Article Title: p53-dependent chromatin relaxation is required for DNA double-strand break repair
    Article Snippet: .. The GFP-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a PEGFP-C1 vector (Addgene, Watertown, USA). .. The GST-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a pGEX-4T-2 vector (Addgene).

    Amplification:

    Article Title: Endoplasmic reticulum exit sites are segregated for secretion based on cargo size.
    Article Snippet: The iLID fragment was originally obtained as a generous gift from the Kuhlman lab (Addgene 60411). .. TANGO1Short was amplified using 5’- GATCCGCTAGCGCTACCGGTGCCACCATGGACTCAGTACCTGCC-3’ and 5’-ACTACTACCACTACTACCTGGGCTCTGTTTTAAAGCCTG-3’, mCherry-iLID was amplified using 5’- GGTAGTAGTGGTAGTAGTATGGTGAGCAAGGGCGA-3’ and 5’- TCGAAGCTTGAGCTCGAGATCTTTAAAAGTAATTTTCGTCGTTCGCT3’.The fragments were inserted into a pEGFP-C1 vector (Addgene 46956) using the AgeI/BglII restriction sites. ..

    Clone Assay:

    Article Title: Mycobacterium tuberculosis modulates NUDT21-mediated alternative polyadenylation to enhance FTH1 expression in macrophages and promotes intracellular growth
    Article Snippet: .. The 3′UTRs of FTH1 transcripts were cloned into a pEGFP-C1 vector (Addgene, Plasmid #36412) downstream of the GFP ORF to create 3′UTR-Long (L) and 3′UTR-Short (S) plasmids. ..

    Construct:

    Article Title: p53-dependent chromatin relaxation is required for DNA double-strand break repair.
    Article Snippet: .. The GFP-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a PEGFP-C1 vector (Addgene, Watertown, USA). .. The GST-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a pGEX-4T-2 vector (Addgene).

    Article Title: p53-dependent chromatin relaxation is required for DNA double-strand break repair
    Article Snippet: .. The GFP-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a PEGFP-C1 vector (Addgene, Watertown, USA). .. The GST-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a pGEX-4T-2 vector (Addgene).

    Cloning:

    Article Title: p53-dependent chromatin relaxation is required for DNA double-strand break repair.
    Article Snippet: .. The GFP-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a PEGFP-C1 vector (Addgene, Watertown, USA). .. The GST-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a pGEX-4T-2 vector (Addgene).

    Article Title: p53-dependent chromatin relaxation is required for DNA double-strand break repair
    Article Snippet: .. The GFP-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a PEGFP-C1 vector (Addgene, Watertown, USA). .. The GST-p53 plasmid was constructed by cloning the full-length cDNA of p53 into a pGEX-4T-2 vector (Addgene).



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